Journal: Molecular Therapy. Methods & Clinical Development
Article Title: rAAV2-Retro Enables Extensive and High-Efficient Transduction of Lower Motor Neurons following Intramuscular Injection
doi: 10.1016/j.omtm.2019.11.006
Figure Lengend Snippet: Comparison of the Transduction Efficiency after the Intramuscular Delivery of Eight Serotypes of rAAV (A–H) Native GFP-expressing cells in transverse cervical spinal cord sections following retrograde transport with recombinant adeno-associated virus (rAAV)-CMV-GFP injection to extensor carpi muscle of the right forelimb of P4 mice (scale bars, 500 μm). (A) rAAV1, (B) rAAV2, (C) rAAV5, (D) rAAV6, (E) rAAV7, (F) rAAV8, (G) rAAV9, and (H) rAAV serotype retro (rAAV-retro). (I) Magnification of (H) (scale bar, 100 μm). (J) Total number of native GFP-expressing cells in the ipsilateral cervical spinal cord. rAAV2-retro demonstrates significantly more efficient retrograde infection than do other serotypes. Statistical analysis of fluorescence measurements is shown in the bar graphs. **p < 0.01, ***p < 0.001, one-way ANOVA followed by the Bonferroni correction (error bars indicate mean ± SEM; n = 4/group). n.s., not significant.
Article Snippet: rAAV serotypes (rAAV1, rAAV2, rAAV5, rAAV6, rAAV7, rAAV8, rAAV9, and rAAV2-retro) were purchased from Vigene Biosciences (Jinan, China).
Techniques: Comparison, Transduction, Expressing, Recombinant, Virus, Injection, Infection, Fluorescence